| Parameter | Range | Optimum |
| Volume of KMnO4 | 0.1 - 1.5 | 0.9 ml |
| Volume of NaOH | 0.1 - 1.5 | 1.1 ml |
| Volume of Henna | 0.1 - 1.5 | 0.5 ml |
| Volume reagent | 0.1 - 1.5 ml | 0.6 ml |
| Reaction time | 10 min | 10 min |